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KMID : 0043319960190020132
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Archives of Pharmacal Research
1996 Volume.19 No. 2 p.132 ~ p.136
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Effects of Oxygen Free Radicals on Extracellular Glutamate Accumulation in Cultured Cells
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Shin Chang-Sik
Oh Sei-Kwan
Oh Ki-Wan
Lee Myung-Koo
Kim Hack-Seang
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Abstract
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Exogenously applied oxygen free radical generating agent, pyrogallol, highly elevated extracellular glutamate accumulation and augmented N-methyl-D-aspartate (NMDA)-induced glutamate accumulation in cerebellar granule neuronal cells, but did not in astrocytes. Superoxide dismutase remarkably decreased the pyrogallol-induced glutamate accumulation, but either NMDA or kainate antagonists did not. In addition, pyrogallol did not affect the NMDAinduced intracellular calcium elevation. Pyrogallol partially blocked glutamate uptake into astrocytes. These results suggest that oxygen free radicals elevate extracellular glutamate accumulation by stimulating the release of glutamate as well as blocking the glutamate uptake.
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KEYWORD
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Pyrogallol, Oxygen free radicals, N-Methyl-D-aspartate, Glutamate accmulation, intracellular calcium, Cerberllar granule neuron, Astrocyte
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